| 场所 | 日期 （年–月） | 病例数（例） | 平均年龄（岁） | 不同症状病例数（例）和百分比（%） | 采样检测数（份） | 检测阳性数（份）和阳性率（%） | 测序标本 | ||
| 发热 | 呕吐 | 腹泻 | |||||||
| 小学1 | 2013–12 | 26 | 7 | 2（7.7） | 26（100.0） | 6（23.0） | 9 | 5（55.6） | N13133 |
| 幼儿园1 | 2014–04 | 14 | 4 | 0（0.0） | 13（92.9） | 4（28.6） | 6 | 4（66.7） | N14024 |
| 幼儿园2 | 2014–11 | 11 | 4 | 0（0.0） | 11（100.0） | 3（27.3） | 5 | 2（40.0） | N14042 |
| 小学2 | 2019–03 | 24 | 10 | 1（4.2） | 22（91.7） | 4（16.7） | 8 | 5（62.5） | N19001 |
| 合计 | 75 | 3（4.0） | 72（96.0） | 17（22.7） | 28 | 16（57.1） | |||
引用本文:
纪蕾, 刘光涛, 沈月华, 查赟峰, 徐德顺. 2013－2019年浙江省湖州市急性胃肠炎病例GII.P7-GII.6型诺如病毒基因特征分析[J]. 疾病监测.
Citation: Lei Ji, Guangtao Liu, Yunfeng Zhu, Yuehua Shen and Deshun Xu. Genetic characteristics of norovirus GII.P7-GII.6 isolated from acute gastroenteritis cases in Huzhou of Zhejiang, 2013–2019[J]. Disease Surveillance.
Citation: Lei Ji, Guangtao Liu, Yunfeng Zhu, Yuehua Shen and Deshun Xu. Genetic characteristics of norovirus GII.P7-GII.6 isolated from acute gastroenteritis cases in Huzhou of Zhejiang, 2013–2019[J]. Disease Surveillance.
2013－2019年浙江省湖州市急性胃肠炎病例GⅡ.P7-GⅡ.6型诺如病毒基因特征分析
摘要:
目的分析2013 — 2019年浙江省湖州市急性胃肠炎病例诺如病毒基因特征，为疾病监测和防控提供参考。 方法采用荧光定量反转录聚合酶链式反应（RT-PCR）方法对2013年12月、2014年4、12月、2019年3月发生的4起学校/托幼机构急性胃肠炎患者送检粪便标本进行诺如病毒核酸检测。 采用RT-PCR法对核酸阳性标本的多聚酶和衣壳蛋白部分区域进行扩增，并对扩增产物进行序列测定。 利用在线分型工具和系统进化分析对病原序列进行基因特征分析。 结果共计28份标本中分别有5、4、2、5份为GⅡ型诺如病毒核酸阳性，阳性率为57.1%（16/28）。 4次疫情各有1份标本测序成功；在线分型和系统进化分支分析显示，4起疫情的病原均为GⅡ.P7-GⅡ.6重组型诺如病毒，但进化分支不同，其中2013年疫情标本检出GⅡ.P7-GⅡ.6c型；2014年2起疫情均为GⅡ.P7-GⅡ.6b型，2019年疫情中检出的GⅡ.P7-GⅡ.6a型。 结论GⅡ.P7-GⅡ.6型重组型诺如病毒是引起湖州市2013 — 2019年4起急性胃肠炎暴发疫情的病原体，每年毒株存在一定基因进化分支的差异。 鉴于该病毒在全球范围内具有持续和广泛的流行能力，应进一步加强对该型别重组型诺如病毒的监测。
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关键词:
- 诺如病毒 /
- 胃肠炎 /
- 暴发 /
- GⅡ.P7-GⅡ.6型
English
Genetic characteristics of norovirus GⅡ.P7-GⅡ.6 isolated from acute gastroenteritis cases in Huzhou of Zhejiang, 2013–2019
Abstract:
ObjectiveTo understand the genetic characteristics of norovirus GⅡ isolated from acute gastroenteritis cases in Huzhou in recent years, and provide reference for disease surveillance and prevention. MethodsFluorescence quantitative RT-PCR was used to detect norovirus nucleic acid in stool samples collected from 4 acute gastroenteritis outbreak in schools and child care settings from 2013 to 2019. RT-PCR was used to amplify the norovirus RNA polymerase and capsid genes of positive samples. The genotype of norovirus was confirmed by phylogenetic analysis and online typing tools. ResultsFour outbreaks of acute gastroenteritis occurred in December 2013, April and December 2014, and March 2019, respectively. A total of 28 samples were collected. Among the samples collected from 4 gastroenteritis outbreaks, 5, 4, 2 and 5 samples were positive for nucleic acid of norovirus GⅡ, and 1 strain from the positive samples was sequenced successfully in each outbreak. Online subtyping and phylogenetic analysis confirmed that the pathogens of the 4 outbreaks were all recombinant norovirus GⅡ.P7-GⅡ.6, but the evolutionary branches were different. The strains detected in 2013 belonged to GⅡ.P7-GⅡ.6c; the strains detected in 2014 belonged to GⅡ.P7-GⅡ.6b; the strains detected in 2019 belonged to GⅡ.P7-GⅡ.6a. ConclusionRecombinant norovirus GⅡ.P7-GⅡ.6 was the pathogen causing 4 outbreaks of acute gastroenteritis in Huzhou from 2013 to 2019, and there were differences in genetic evolutionary branches among the strains isolated in different years. Given the sustainable and wide spread of norovirus GⅡ.P7-GⅡ.6 worldwide, the surveillance for this type of recombinant norovirus should be further strengthened.
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Key words:
- Norovirus /
- Gastroenteritis /
- Outbreak /
- GⅡ.P7-GⅡ.6
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图 1 诺如病毒衣壳蛋白片段的系统进化分析
Figure 1. Phylogenetic analysis based on partial capsid gene sequences of norovirus
图 2 诺如病毒多聚酶区片段的系统进化分析
Figure 2. Phylogenetic analysis based on partial RdRp gene sequences of norovirus
图 3 诺如病毒RdRp区至衣壳蛋白区的长片段系统进化分析
Figure 3. Phylogenetic analysis based on partial RdRp and capsid gene sequences of norovirus
表 1 湖州市急性胃肠炎病例诺如病毒感染概况
Table 1. Infection status of norovirus in acute gastroenteritis cases in Huzhou
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